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Assay Detail

CHEMBL5164645

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Binding
Inhibition of MDM2/p53 interaction in human U-87 MG cells using TMB as substrate incubated for 10 mins by quantitative immuno-enzymatic assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type U-87 MG
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Tumour suppressor p53/oncoprotein Mdm2 (CHEMBL1907611)
Type PROTEIN-PROTEIN INTERACTION
Organism Homo sapiens

Publication

Structure-Based Discovery of MDM2/4 Dual Inhibitors that Exert Antitumor Activities against MDM4-Overexpressing Cancer Cells.
Zhang S, Yan Z, Li Y, Gong Y, Lyu X, Lou J, Zhang D, Meng X, Zhao Y.
J Med Chem (2022) 65 : 6207 -6230
PubMed 35420431 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 8.04 8.11

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4095983 1 8.11
CHEMBL2313487 1 7.97

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4095983 IC50 = 7.85 nM 8.11
CHEMBL2313487 IC50 = 10.6 nM 7.97