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Assay Detail

CHEMBL5165094

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Reactivation of mouse CTLL-2 cells pretreated with human PD-L1 assessed as increase in cell proliferation and measured by flow cytometry assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type CTLL-2
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Pyrazolones as inhibitors of immune checkpoint blocking the PD-1/PD-L1 interaction.
Le Biannic R, Magnez R, Klupsch F, Leleu-Chavain N, Thiroux B, Tardy M, El Bouazzati H, Dezitter X, Renault N, Vergoten G, Bailly C, Quesnel B, Thuru X, Millet R.
Eur J Med Chem (2022) 236 : 114343 -114343
PubMed 35429911 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 7.52 8.39

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5207924 1 8.39
CHEMBL5176505 1 7.85
CHEMBL5194957 1 7.27
CHEMBL5171729 1 7.12
CHEMBL374252 1 6.99

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5207924 IC50 = 4.1 nM 8.39
CHEMBL5176505 IC50 = 14.0 nM 7.85
CHEMBL5194957 IC50 = 54.0 nM 7.27
CHEMBL5171729 IC50 = 75.0 nM 7.12
CHEMBL374252 IC50 = 102.0 nM 6.99