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Assay Detail

CHEMBL5166010

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of eGFP-tagged rat GluN1-1a/GluN2A transfected in human tsA201 cells assessed as inhibition glutamate-induced current measured at -65 mV holding potential applied for 10 secs by whole-cell patch-clamp method
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type tsA201
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Glutamate NMDA receptor; Grin1/Grin2a (CHEMBL2096680)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Design, synthesis, and in vitro and in vivo characterization of new memantine analogs for Alzheimer's disease.
Turcu AL, Companys-Alemany J, Phillips MB, Patel DS, Griñán-Ferré C, Loza MI, Brea JM, Pérez B, Soto D, Sureda FX, Kurnikova MG, Johnson JW, Pallàs M, Vázquez S.
Eur J Med Chem (2022) 236 : 114354 -114354
PubMed 35453065 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.44 6.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL807 MEMANTINE 4.0 1 6.00
CHEMBL742 KETAMINE 4.0 1 6.00
CHEMBL5187677 1 5.36
CHEMBL660 AMANTADINE 4.0 1 4.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL807 MEMANTINE IC50 = 1000.0 nM 6.00
CHEMBL742 KETAMINE IC50 = 1000.0 nM 6.00
CHEMBL5187677 IC50 = 4400.0 nM 5.36
CHEMBL660 AMANTADINE IC50 = 40000.0 nM 4.40