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Assay Detail

CHEMBL5166090

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ERK5 phosphorylation in human HeLa cells incubated for 1 hr by Western blot densitometry analysis
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase 7 (CHEMBL5332)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Parallel Optimization of Potency and Pharmacokinetics Leading to the Discovery of a Pyrrole Carboxamide ERK5 Kinase Domain Inhibitor.
Miller DC, Reuillon T, Molyneux L, Blackburn T, Cook SJ, Edwards N, Endicott JA, Golding BT, Griffin RJ, Hardcastle I, Harnor SJ, Heptinstall A, Lochhead P, Martin MP, Martin NC, Myers S, Newell DR, Noble RA, Phillips N, Rigoreau L, Thomas H, Tucker JA, Wang LZ, Waring MJ, Wong AC, Wedge SR, Noble MEM, Cano C.
J Med Chem (2022) 65 : 6513 -6540
PubMed 35468293 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 7.04 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5197199 1 7.70
CHEMBL5186248 1 7.51
CHEMBL5173346 1 7.37
CHEMBL5175760 1 7.24
CHEMBL5172952 1 7.22
CHEMBL5188920 1 7.16
CHEMBL5193786 1 6.85
CHEMBL5198962 1 6.17
CHEMBL5180089 1 6.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5197199 IC50 = 20.0 nM 7.70
CHEMBL5186248 IC50 = 31.0 nM 7.51
CHEMBL5173346 IC50 = 43.0 nM 7.37
CHEMBL5175760 IC50 = 58.0 nM 7.24
CHEMBL5172952 IC50 = 60.0 nM 7.22
CHEMBL5188920 IC50 = 70.0 nM 7.16
CHEMBL5193786 IC50 = 141.0 nM 6.85
CHEMBL5198962 IC50 = 671.0 nM 6.17
CHEMBL5180089 IC50 = 787.0 nM 6.10