Assay Detail
Binding
CHEMBL5166487
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to recombinant human CaMK2alpha Phe467Met mutant expressed in Escherichia coli BL21 DE3 assessed as dissociation constant by surface plasmon resonance assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Calcium/calmodulin-dependent protein kinase type II subunit alpha (CHEMBL4147) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Discovery and Optimization of 5-Hydroxy-Diclofenac toward a New Class of Ligands with Nanomolar Affinity for the CaMKIIα Hub Domain.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 4 | 6.49 | 6.66 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5191835 | — | — | 1 | 6.66 |
| CHEMBL5196669 | — | — | 1 | 6.66 |
| CHEMBL5181927 | — | — | 1 | 6.45 |
| CHEMBL5198167 | — | — | 1 | 6.17 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5191835 | — | Kd | = | 219.0 | nM | 6.66 |
| CHEMBL5196669 | — | Kd | = | 219.0 | nM | 6.66 |
| CHEMBL5181927 | — | Kd | = | 353.0 | nM | 6.45 |
| CHEMBL5198167 | — | Kd | = | 670.0 | nM | 6.17 |