Assay Detail
Functional
CHEMBL5209600
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of the Dopamine transporter (DAT, SLC6A3) as assessed by GPCR-mediated changes in cell morphology using the impedance-based transporter activity through receptor activation (TRACT) assay in HEK-293 JumpIN-SLC6A3 cells (PubChem AID: 1745858)
3
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Sodium-dependent dopamine transporter (CHEMBL238) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
A study of the dopamine transporter using the TRACT assay, a novel in vitro tool for solute carrier drug discovery
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 6.46 | 6.70 |
| EC50 | 1 | 4.34 | 4.34 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL543876 | — | — | 1 | 6.70 |
| CHEMBL529437 | COCAINE HYDROCHLORIDE | 4.0 | 1 | 6.22 |
| CHEMBL1557 | DOPAMINE HYDROCHLORIDE | 4.0 | 1 | 4.34 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL543876 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL529437 | COCAINE HYDROCHLORIDE | IC50 | = | 600.0 | nM | 6.22 |
| CHEMBL1557 | DOPAMINE HYDROCHLORIDE | EC50 | = | 46000.0 | nM | 4.34 |