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Assay Detail

CHEMBL5209601

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Substrate uptake and inhibition of the chromaffin granule amine transporter (VAT1, VMAT1, SLC18A1) as assessed by uptake of a fluorescent substrate (FFN206) in HEK-293 JumpIN-SLC18A1 cells (PubChem AID: 1794818)
3
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Chromaffin granule amine transporter (CHEMBL1838)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

FFN206 based assay for SLC18A1 using HEK-293 SLC18A1 OE cells
Sara Tremolada; Loredana Redaelli; Lia Scarabottolo
(2022)
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 7.30 7.30
EC50 1 5.70 5.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL772 RESERPINE 4.0 1 7.30
CHEMBL5209629 1 5.70
CHEMBL117785 TETRABENAZINE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL772 RESERPINE IC50 = 50.0 nM 7.30
CHEMBL5209629 EC50 = 2000.0 nM 5.70
CHEMBL117785 TETRABENAZINE IC50 > 10000.0 nM -