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Assay Detail

CHEMBL5209602

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Substrate uptake and inhibition of the Vesicular Amine Transporter 2 (VMAT2, SLC18A2) as assessed by uptake of a fluorescent substrate (FFN206) in HEK-293 JumpIN-SLC18A2 cells (PubChem AID: 1794817)
3
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Synaptic vesicular amine transporter (CHEMBL1893)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

FFN206 based assay for SLC18A2 using HEK-293 SLC18A2 OE cells
Sara Tremolada; Loredana Redaelli; Lia Scarabottolo
(2022)
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 7.76 7.81
EC50 1 5.45 5.45

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL772 RESERPINE 4.0 1 7.81
CHEMBL117785 TETRABENAZINE 4.0 1 7.70
CHEMBL5209629 1 5.45

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL772 RESERPINE IC50 = 15.5 nM 7.81
CHEMBL117785 TETRABENAZINE IC50 = 19.8 nM 7.70
CHEMBL5209629 EC50 = 3560.0 nM 5.45