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Assay Detail

CHEMBL5209624

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of the Norepinephrine transporter (NET, SLC6A2) as assessed by GPCR-mediated changes in cell morphology using the impedance-based transporter activity through receptor activation (TRACT) assay in HEK-293 JumpIN-SLC6A2 cells (PubChem AID: 1745860)
15
Total Activities
15
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium-dependent noradrenaline transporter (CHEMBL222)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Label-free high-throughput screening assay for the identification of norepinephrine transporter (NET/SLC6A2) inhibitors
Hubert J. Sijben; Wieke M. van Oostveen; Peter B. R. Hartog; Laura Stucchi; Andrea Rossignoli; Giovanna Maresca; Lia Scarabottolo; Adriaan P. IJzerman; Laura H. Heitman
Sci Rep (2021) 11 : 12290
PubMed 34112854 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 7.08 8.30
EC50 3 5.10 5.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1256695 NISOXETINE HYDROCHLORIDE 1 8.30
CHEMBL2105282 1 8.30
CHEMBL1696 DESIPRAMINE HYDROCHLORIDE 4.0 1 8.20
CHEMBL1702 ATOMOXETINE HYDROCHLORIDE 4.0 1 7.70
CHEMBL1201156 NORTRIPTYLINE HYDROCHLORIDE 4.0 1 7.40
CHEMBL1237135 MAPROTILINE HYDROCHLORIDE 4.0 1 7.30
CHEMBL1237129 1R,2S-MILNACIPRAN HYDROCHLORIDE 1 7.30
CHEMBL1200964 AMITRIPTYLINE HYDROCHLORIDE 4.0 1 6.70
CHEMBL85236 1 6.50
CHEMBL529437 COCAINE HYDROCHLORIDE 4.0 1 6.20
CHEMBL281594 VANOXERINE 3.0 1 6.10
CHEMBL1200818 1 5.40
CHEMBL5087452 1 5.20
CHEMBL1698 BUPROPION HYDROCHLORIDE 4.0 1 4.90
CHEMBL1557 DOPAMINE HYDROCHLORIDE 4.0 1 4.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1256695 NISOXETINE HYDROCHLORIDE IC50 = 5.0 nM 8.30
CHEMBL2105282 IC50 = 5.0 nM 8.30
CHEMBL1696 DESIPRAMINE HYDROCHLORIDE IC50 = 6.3 nM 8.20
CHEMBL1702 ATOMOXETINE HYDROCHLORIDE IC50 = 20.0 nM 7.70
CHEMBL1201156 NORTRIPTYLINE HYDROCHLORIDE IC50 = 40.0 nM 7.40
CHEMBL1237135 MAPROTILINE HYDROCHLORIDE IC50 = 50.0 nM 7.30
CHEMBL1237129 1R,2S-MILNACIPRAN HYDROCHLORIDE IC50 = 50.0 nM 7.30
CHEMBL1200964 AMITRIPTYLINE HYDROCHLORIDE IC50 = 200.0 nM 6.70
CHEMBL85236 IC50 = 320.0 nM 6.50
CHEMBL529437 COCAINE HYDROCHLORIDE IC50 = 630.0 nM 6.20
CHEMBL281594 VANOXERINE IC50 = 790.0 nM 6.10
CHEMBL1200818 EC50 = 4000.0 nM 5.40
CHEMBL5087452 EC50 = 6300.0 nM 5.20
CHEMBL1698 BUPROPION HYDROCHLORIDE IC50 = 12600.0 nM 4.90
CHEMBL1557 DOPAMINE HYDROCHLORIDE EC50 = 20000.0 nM 4.70