Assay Detail
Functional
CHEMBL5209947
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Affinity Phenotypic Cellular interaction (High Content Imaging Mitotic Index Assay (increase of mitotic index based on phospho-histone H3 expression in HeLa cells)) EUB0000665a CHEK1
1
Total Activities
1
Compounds Tested
1
Activity Types
3
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Category | Affinity phenotypic cellular assay |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Serine/threonine-protein kinase Chk1 (CHEMBL4630) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Affinity Phenotypic Cellular Literature for EUbOPEN Chemogenomics Library wave 3
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 1 | 7.42 | 7.42 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| Mode of action | Mode of action | - | — | Inhibitor |
| Protein family | Protein family | - | — | Protein Kinase |
| Recommended concentration | Recommended concentration | 100.0 | nM | — |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3990456 | — | — | 1 | 7.42 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3990456 | — | EC50 | = | 38.0 | nM | 7.42 |