Assay Detail
Binding
CHEMBL5216876
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Thermus thermophilus RuvA/RuvB protein complex helicase activity expressed in Escherichia coli BL21(DE3) assessed as inhibition of HJ branch migration preincubated for 15 mins followed by substrate addition and measured after 1 hr using 5'-FAM labelled 5'-CACTGTGATGCACGATGATTGACGACAGTAGTCAGTGCTGCAGTGGTCAGGTGTC-3' HJ-a duplex DNA as substrate in presence of ATP by PAGE analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Thermus thermophilus |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Identification of small-molecule inhibitors of the DNA repair proteins RuvAB from Pseudomonas aeruginosa.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 5.53 | 5.96 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL449392 | CORILAGIN | — | 1 | 5.96 |
| CHEMBL5219130 | — | — | 1 | 5.10 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL449392 | CORILAGIN | IC50 | = | 1110.0 | nM | 5.96 |
| CHEMBL5219130 | — | IC50 | = | 7900.0 | nM | 5.10 |