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Assay Detail

CHEMBL5217045

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Stabilization of mode-1 phosphopeptide FAM-KRSHpSVP binding to recombinant 14-3-3 zeta (unknown origin) measured by fluorescence polarization assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Structure-activity-relationship study of semi-synthetically modified fusicoccins on their stabilization effect for 14-3-3-phospholigand interactions.
Ogino N, Masuda R, Lynn Punzalan L, Yamashita E, Igaue S, Inoue Y, Ohkanda J.
Bioorg Med Chem (2022) 73 : 117020 -117020
PubMed 36182801 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 20 6.51 7.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5220154 1 7.22
CHEMBL5220954 1 7.10
CHEMBL5220627 1 6.96
CHEMBL5219556 1 6.96
CHEMBL5219891 1 6.89
CHEMBL5218877 1 6.80
CHEMBL5221089 1 6.77
CHEMBL4218471 1 6.64
CHEMBL5220966 1 6.51
CHEMBL5221051 1 6.37
CHEMBL5220019 1 6.33
CHEMBL5218483 1 6.27
CHEMBL5219325 1 6.21
CHEMBL5218751 1 6.19
CHEMBL5219378 1 6.18
CHEMBL5218600 1 6.17
CHEMBL5219992 1 6.17
CHEMBL5220189 1 6.17
CHEMBL5220857 1 6.15
CHEMBL5219448 1 6.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5220154 Kd = 60.0 nM 7.22
CHEMBL5220954 Kd = 80.0 nM 7.10
CHEMBL5220627 Kd = 110.0 nM 6.96
CHEMBL5219556 Kd = 110.0 nM 6.96
CHEMBL5219891 Kd = 130.0 nM 6.89
CHEMBL5218877 Kd = 160.0 nM 6.80
CHEMBL5221089 Kd = 170.0 nM 6.77
CHEMBL4218471 Kd = 230.0 nM 6.64
CHEMBL5220966 Kd = 310.0 nM 6.51
CHEMBL5221051 Kd = 430.0 nM 6.37
CHEMBL5220019 Kd = 470.0 nM 6.33
CHEMBL5218483 Kd = 540.0 nM 6.27
CHEMBL5219325 Kd = 620.0 nM 6.21
CHEMBL5218751 Kd = 650.0 nM 6.19
CHEMBL5219378 Kd = 660.0 nM 6.18
CHEMBL5218600 Kd = 680.0 nM 6.17
CHEMBL5219992 Kd = 680.0 nM 6.17
CHEMBL5220189 Kd = 680.0 nM 6.17
CHEMBL5220857 Kd = 710.0 nM 6.15
CHEMBL5219448 Kd = 750.0 nM 6.12