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Assay Detail

CHEMBL5217075

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of hedgehog signalling pathway in human A549 cells assessed as reduction in Gli gene expression incubated for 24 hrs by qPCR analysis
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type A549
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Design, synthesis and activity evaluation of Hedgehog inhibitor Itraconazole derivatives in A549 cells.
Cai J, Chen X, You H, Li X, Ji M.
Bioorg Med Chem Lett (2022) 76 : 129011 -129011
PubMed 36184028 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 6.45 7.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5219278 1 7.05
CHEMBL64391 ITRACONAZOLE 4.0 1 6.90
CHEMBL5219499 1 6.60
CHEMBL5218534 1 6.34
CHEMBL5219258 1 6.11
CHEMBL5219088 1 6.07
CHEMBL5219502 1 6.06

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5219278 IC50 = 89.0 nM 7.05
CHEMBL64391 ITRACONAZOLE IC50 = 125.0 nM 6.90
CHEMBL5219499 IC50 = 251.0 nM 6.60
CHEMBL5218534 IC50 = 457.0 nM 6.34
CHEMBL5219258 IC50 = 782.0 nM 6.11
CHEMBL5219088 IC50 = 844.0 nM 6.07
CHEMBL5219502 IC50 = 873.0 nM 6.06