Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5218090

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat ADCY10 overexpressed in rat 4-4 cells assessed as IBMX stimulated cAMP accumulation preincubated for 10 mins followed by IBMX stimulation and measured after 5 mins by ELISA
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type 4-4 cell
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenylate cyclase type 10 (CHEMBL5169208)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Design, Synthesis, and Pharmacological Evaluation of Second-Generation Soluble Adenylyl Cyclase (sAC, ADCY10) Inhibitors with Slow Dissociation Rates.
Miller M, Rossetti T, Ferreira J, Ghanem L, Balbach M, Kaur N, Levin LR, Buck J, Kehr M, Coquille S, van den Heuvel J, Steegborn C, Fushimi M, Finkin-Groner E, Myers RW, Kargman S, Liverton NJ, Huggins DJ, Meinke PT.
J Med Chem (2022) 65 : 15208 -15226
PubMed 36346696 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.97 8.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5218698 1 8.72
CHEMBL5220685 1 8.60
CHEMBL5218878 1 8.26
CHEMBL5219830 1 8.26
CHEMBL5220647 1 7.97
CHEMBL5218615 1 7.84
CHEMBL5218702 1 7.78
CHEMBL5218552 1 7.71
CHEMBL5219443 1 7.64
CHEMBL5219579 1 7.46
CHEMBL5220632 1 7.42

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5218698 IC50 = 1.9 nM 8.72
CHEMBL5220685 IC50 = 2.5 nM 8.60
CHEMBL5218878 IC50 = 5.5 nM 8.26
CHEMBL5219830 IC50 = 5.5 nM 8.26
CHEMBL5220647 IC50 = 10.8 nM 7.97
CHEMBL5218615 IC50 = 14.6 nM 7.84
CHEMBL5218702 IC50 = 16.7 nM 7.78
CHEMBL5218552 IC50 = 19.6 nM 7.71
CHEMBL5219443 IC50 = 22.9 nM 7.64
CHEMBL5219579 IC50 = 34.8 nM 7.46
CHEMBL5220632 IC50 = 37.7 nM 7.42