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Assay Detail

CHEMBL5224110

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant His-tagged mouse KIF18A expressed in Trichoplusia ni insect cells assessed as inhibition of microtubule-stimulated ATPase activity preincubated for 30 mins followed by ATP addition and further incubated for 15 mins by ADP-Glo reagent based assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type Insect cell
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kinesin-like protein KIF18A (CHEMBL5291576)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Targeting the Mitotic Kinesin KIF18A in Chromosomally Unstable Cancers: Hit Optimization Toward an In Vivo Chemical Probe.
Tamayo NA, Bourbeau MP, Allen JR, Ashton KS, Chen JJ, Kaller MR, Nguyen TT, Nishimura N, Pettus LH, Walton M, Belmontes B, Moriguchi J, Chen K, McCarter JD, Hanestad K, Chung G, Ninniri MSS, Sun J, Poppe L, Spahr C, Hui J, Jia L, Wu T, Dahal UP, Edson KZ, Payton M.
J Med Chem (2022) 65 : 4972 -4990
PubMed 35286090 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.11 7.11

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5287177 1 7.11

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5287177 IC50 = 77.0 nM 7.11