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Assay Detail

CHEMBL5224837

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiproliferative activity against human NCI-H1299 cells assessed as cell growth inhibition incubated for 3 days by CellTiter-Glo Luminescent assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type NCI-H1299
Confidence 1 — Organism
Curated By Autocuration

Target

NCI-H1299 (CHEMBL612255)
Type CELL-LINE
Organism Homo sapiens

Publication

Discovery of pyrrolo[2,3-d]pyrimidine-based molecules as a Wee1 inhibitor template.
Chen C, Wang Y, Hu MQ, Li H, Chen X, Qiang G, Sun Y, Zhu Y, Li B.
Bioorg Med Chem Lett (2022) 75 : 128973 -128973
PubMed 36075370 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 6.93 7.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5284925 1 7.64
CHEMBL5290439 1 7.48
CHEMBL5289843 1 7.38
CHEMBL5282382 1 7.38
CHEMBL5284989 1 7.37
CHEMBL5278196 1 7.27
CHEMBL5269524 1 7.23
CHEMBL5281278 1 7.17
CHEMBL5279297 1 7.06
CHEMBL5279054 1 7.04
CHEMBL5278751 1 7.00
CHEMBL5288235 1 7.00
CHEMBL1976040 ADAVOSERTIB 2.0 1 6.98
CHEMBL5279737 1 6.98
CHEMBL5273079 1 6.90
CHEMBL5279123 1 6.81
CHEMBL5199347 1 6.70
CHEMBL5270034 1 6.68
CHEMBL5267467 1 6.54
CHEMBL5267186 1 6.48
CHEMBL5290782 1 6.35
CHEMBL5192067 1 6.21
CHEMBL5288823 1 5.76

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5284925 IC50 = 23.0 nM 7.64
CHEMBL5290439 IC50 = 33.0 nM 7.48
CHEMBL5289843 IC50 = 42.0 nM 7.38
CHEMBL5282382 IC50 = 42.0 nM 7.38
CHEMBL5284989 IC50 = 43.0 nM 7.37
CHEMBL5278196 IC50 = 54.0 nM 7.27
CHEMBL5269524 IC50 = 59.0 nM 7.23
CHEMBL5281278 IC50 = 67.0 nM 7.17
CHEMBL5279297 IC50 = 87.0 nM 7.06
CHEMBL5279054 IC50 = 91.0 nM 7.04
CHEMBL5278751 IC50 = 100.0 nM 7.00
CHEMBL5288235 IC50 = 101.0 nM 7.00
CHEMBL1976040 ADAVOSERTIB IC50 = 104.0 nM 6.98
CHEMBL5279737 IC50 = 105.0 nM 6.98
CHEMBL5273079 IC50 = 126.0 nM 6.90
CHEMBL5279123 IC50 = 156.0 nM 6.81
CHEMBL5199347 IC50 = 199.0 nM 6.70
CHEMBL5270034 IC50 = 211.0 nM 6.68
CHEMBL5267467 IC50 = 288.0 nM 6.54
CHEMBL5267186 IC50 = 332.0 nM 6.48
CHEMBL5290782 IC50 = 451.0 nM 6.35
CHEMBL5192067 IC50 = 623.0 nM 6.21
CHEMBL5288823 IC50 = 1719.0 nM 5.76