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Assay Detail

CHEMBL5225883

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Anti-osteoclastogensis activity in BALB/c mouse bone marrow macrophage cells assessed as inhibition of RANKL/M-CSF-induced osteoclastogenesis by measuring osteoclasts number incubated for 11 days by TRAP staining based assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type Macrophage
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Synthesis and biological evaluation of pyrazole-fused oleanolic acid derivatives as novel inhibitors of inflammatory and osteoclast differentiation.
Yu Y, Yuan W, Yuan J, Wei W, He Q, Zhang X, He S, Yang C.
Bioorg Med Chem (2023) 80 : 117177 -117177
PubMed 36701870 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.33 5.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5270204 1 5.59
CHEMBL5265923 1 5.57
CHEMBL5290776 1 5.57
CHEMBL5277425 1 5.42
CHEMBL5275936 1 5.39
CHEMBL5266874 1 5.30
CHEMBL5273423 1 5.07
CHEMBL168 OLEANOLIC_ACID 1 4.73

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5270204 IC50 = 2550.0 nM 5.59
CHEMBL5265923 IC50 = 2720.0 nM 5.57
CHEMBL5290776 IC50 = 2720.0 nM 5.57
CHEMBL5277425 IC50 = 3820.0 nM 5.42
CHEMBL5275936 IC50 = 4100.0 nM 5.39
CHEMBL5266874 IC50 = 4950.0 nM 5.30
CHEMBL5273423 IC50 = 8600.0 nM 5.07
CHEMBL168 OLEANOLIC_ACID IC50 = 18640.0 nM 4.73