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Assay Detail

CHEMBL5226037

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of 6His-tagged SOS1 (unknown origin) mediated GST-tagged KRAS G12D activation preincubated for 15 mins followed by KRAS G12D addition and measured after 30 mins by HTRF assay
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Son of sevenless homolog 1 (CHEMBL2079846)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, and Bioevaluation of Pyrido[2,3-<i>d</i>]pyrimidin-7-ones as Potent SOS1 Inhibitors.
Liu M, Zhou G, Su W, Gu Y, Gao M, Wang K, Huo R, Li Y, Zhou Z, Chen K, Zheng M, Zhang S, Xu T.
ACS Med Chem Lett (2023) 14 : 183 -190
PubMed 36793426 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 6.84 7.17

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5275216 1 7.17
CHEMBL5280533 1 7.15
CHEMBL4519023 1 7.14
CHEMBL5290547 1 7.14
CHEMBL5286601 1 7.13
CHEMBL5269963 1 7.11
CHEMBL5284292 1 7.07
CHEMBL5266985 1 7.06
CHEMBL5267612 1 7.04
CHEMBL5280431 1 7.03
CHEMBL5275839 1 7.03
CHEMBL5274267 1 7.02
CHEMBL5279147 1 6.87
CHEMBL5266146 1 6.86
CHEMBL5282775 1 6.77
CHEMBL5279160 1 6.65
CHEMBL5287813 1 6.33
CHEMBL5281607 1 6.16
CHEMBL5271901 1 6.10
CHEMBL5275170 1 6.06
CHEMBL5288876 1 -
CHEMBL5270449 1 -
CHEMBL5288585 1 -
CHEMBL5290546 1 -
CHEMBL5270088 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5275216 IC50 = 67.4 nM 7.17
CHEMBL5280533 IC50 = 70.5 nM 7.15
CHEMBL4519023 IC50 = 71.8 nM 7.14
CHEMBL5290547 IC50 = 72.1 nM 7.14
CHEMBL5286601 IC50 = 73.4 nM 7.13
CHEMBL5269963 IC50 = 77.5 nM 7.11
CHEMBL5284292 IC50 = 85.6 nM 7.07
CHEMBL5266985 IC50 = 87.8 nM 7.06
CHEMBL5267612 IC50 = 90.8 nM 7.04
CHEMBL5280431 IC50 = 94.0 nM 7.03
CHEMBL5275839 IC50 = 92.8 nM 7.03
CHEMBL5274267 IC50 = 95.4 nM 7.02
CHEMBL5279147 IC50 = 134.0 nM 6.87
CHEMBL5266146 IC50 = 138.0 nM 6.86
CHEMBL5282775 IC50 = 171.0 nM 6.77
CHEMBL5279160 IC50 = 226.0 nM 6.65
CHEMBL5287813 IC50 = 463.0 nM 6.33
CHEMBL5281607 IC50 = 690.0 nM 6.16
CHEMBL5271901 IC50 = 801.0 nM 6.10
CHEMBL5275170 IC50 = 869.0 nM 6.06
CHEMBL5288876 IC50 > 5000.0 nM -
CHEMBL5270449 IC50 > 5000.0 nM -
CHEMBL5288585 IC50 > 5000.0 nM -
CHEMBL5290546 IC50 > 5000.0 nM -
CHEMBL5270088 IC50 > 5000.0 nM -