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Assay Detail

CHEMBL5226587

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat brain aSMase using HMU-PC as substrate incubated for 3 hrs by fluorescence plate reader assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Small Molecule Inhibitors Targeting Biosynthesis of Ceramide, the Central Hub of the Sphingolipid Network.
Skácel J, Slusher BS, Tsukamoto T.
J Med Chem (2021) 64 : 279 -297
PubMed 33395289 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 7.34 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5288063 1 7.70
CHEMBL4553570 1 7.40
CHEMBL416301 1 7.16
CHEMBL54004 1 7.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5288063 IC50 = 20.0 nM 7.70
CHEMBL4553570 IC50 = 40.0 nM 7.40
CHEMBL416301 IC50 = 70.0 nM 7.16
CHEMBL54004 IC50 = 80.0 nM 7.10