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Assay Detail

CHEMBL5228705

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human FLT3-ITD mutant preincubated with enzyme for 20 mins followed by [gamma-33P]ATP addition and measured after 120 mins by filter binding method
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Receptor-type tyrosine-protein kinase FLT3 (CHEMBL1974)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Potent FLT3 Inhibitor (LT-850-166) with the Capacity of Overcoming a Variety of FLT3 Mutations.
Wang Z, Cai J, Ren J, Chen Y, Wu Y, Cheng J, Jia K, Huang F, Cheng Z, Sheng T, Song S, Heng H, Zhu Y, Tang W, Li H, Lu T, Chen Y, Lu S.
J Med Chem (2021) 64 : 14664 -14701
PubMed 34550682 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 8.30 9.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5289647 1 9.15
CHEMBL5280424 1 9.07
CHEMBL5275773 1 9.03
CHEMBL5267748 1 9.03
CHEMBL5288323 1 8.94
CHEMBL4456471 1 8.87
CHEMBL5273185 1 8.85
CHEMBL4077071 1 8.77
CHEMBL5198364 1 8.73
CHEMBL5278798 1 8.69
CHEMBL5287778 1 8.49
CHEMBL5270580 1 8.45
CHEMBL5279911 1 8.45
CHEMBL5277795 1 8.43
CHEMBL5270690 1 8.42
CHEMBL5289832 1 8.41
CHEMBL5277875 1 8.41
CHEMBL5287986 1 8.34
CHEMBL5290123 1 8.30
CHEMBL5290855 1 8.30
CHEMBL5270966 1 8.24
CHEMBL5287938 1 8.12
CHEMBL5286906 1 8.12
CHEMBL5272321 1 8.08
CHEMBL5280468 1 8.04
CHEMBL5287861 1 7.99
CHEMBL5273225 1 7.95
CHEMBL5281795 1 7.89
CHEMBL5266516 1 7.85
CHEMBL5283600 1 7.79

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5289647 IC50 = 0.7 nM 9.15
CHEMBL5280424 IC50 = 0.85 nM 9.07
CHEMBL5275773 IC50 = 0.94 nM 9.03
CHEMBL5267748 IC50 = 0.94 nM 9.03
CHEMBL5288323 IC50 = 1.14 nM 8.94
CHEMBL4456471 IC50 = 1.34 nM 8.87
CHEMBL5273185 IC50 = 1.42 nM 8.85
CHEMBL4077071 IC50 = 1.71 nM 8.77
CHEMBL5198364 IC50 = 1.84 nM 8.73
CHEMBL5278798 IC50 = 2.06 nM 8.69
CHEMBL5287778 IC50 = 3.22 nM 8.49
CHEMBL5270580 IC50 = 3.59 nM 8.45
CHEMBL5279911 IC50 = 3.58 nM 8.45
CHEMBL5277795 IC50 = 3.72 nM 8.43
CHEMBL5270690 IC50 = 3.77 nM 8.42
CHEMBL5277875 IC50 = 3.86 nM 8.41
CHEMBL5289832 IC50 = 3.88 nM 8.41
CHEMBL5287986 IC50 = 4.58 nM 8.34
CHEMBL5290123 IC50 = 4.96 nM 8.30
CHEMBL5290855 IC50 = 4.96 nM 8.30
CHEMBL5270966 IC50 = 5.73 nM 8.24
CHEMBL5287938 IC50 = 7.65 nM 8.12
CHEMBL5286906 IC50 = 7.59 nM 8.12
CHEMBL5272321 IC50 = 8.34 nM 8.08
CHEMBL5280468 IC50 = 9.06 nM 8.04
CHEMBL5287861 IC50 = 10.33 nM 7.99
CHEMBL5273225 IC50 = 11.13 nM 7.95
CHEMBL5281795 IC50 = 12.9 nM 7.89
CHEMBL5266516 IC50 = 14.06 nM 7.85
CHEMBL5283600 IC50 = 16.05 nM 7.79
CHEMBL5276126 IC50 = 23.7 nM 7.62
CHEMBL5272638 IC50 = 213.3 nM 6.67
CHEMBL5280266 IC50 = 397.3 nM 6.40