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Assay Detail

CHEMBL5228934

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length USP5 (1 to 835 residues) (unknown origin) expressed in Escherichia coli using DU48-03 as substrate preincubated with enzyme for 1 hr followed by substrate addition and measured for 10 mins by IQF assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Ubiquitin carboxyl-terminal hydrolase 5 (CHEMBL6158)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Activity Relationship of USP5 Inhibitors.
Mann MK, Zepeda-Velázquez CA, González-Álvarez H, Dong A, Kiyota T, Aman AM, Loppnau P, Li Y, Wilson B, Arrowsmith CH, Al-Awar R, Harding RJ, Schapira M.
J Med Chem (2021) 64 : 15017 -15036
PubMed 34648286 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 6.10 6.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5278336 1 6.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5278336 IC50 = 800.0 nM 6.10