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Assay Detail

CHEMBL5229424

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Induction of bacterial inner membrane permeabilization in Escherichia coli assessed as carboxyfluorescein leakage measured for 45 mins by fluorescence based microplate reader assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 2 — Organism
Curated By Autocuration

Target

Cell membrane (CHEMBL2364031)
Type SUBCELLULAR
Organism Bacteria

Publication

Screening for Cyclotides in Sri Lankan Medicinal Plants: Discovery, Characterization, and Bioactivity Screening of Cyclotides from <i>Geophila repens</i>.
Rajendran S, Slazak B, Mohotti S, Muhammad T, Strömstedt AA, Kapusta M, Wilmowicz E, Göransson U, Hettiarachchi CM, Gunasekera S.
J Nat Prod (2023) 86 : 52 -65
PubMed 36525646 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 6.93 7.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5271959 1 7.16
CHEMBL4211294 1 7.03
CHEMBL5290770 1 6.85
CHEMBL5283188 1 6.80
CHEMBL5291484 1 6.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5271959 EC50 = 70.0 nM 7.16
CHEMBL4211294 EC50 = 94.0 nM 7.03
CHEMBL5290770 EC50 = 140.0 nM 6.85
CHEMBL5283188 EC50 = 160.0 nM 6.80
CHEMBL5291484 EC50 = 160.0 nM 6.80