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Assay Detail

CHEMBL5229426

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at PPARgamma in human BMMSC cells assessed as promotion of adiponectin secretion by measuring increase in adiponectin level in presence of IDX medium by ELISA
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type BMMSC
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor gamma (CHEMBL235)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Alkaloids and Coumarins with Adiponectin-Secretion-Promoting Activities from the Leaves of <i>Orixa japonica</i>.
Nhoek P, Ahn S, Pel P, Kim YM, Huh J, Kim HW, Noh M, Chin YW.
J Nat Prod (2023) 86 : 138 -148
PubMed 36529937 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 5.33 6.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL595 PIOGLITAZONE 4.0 1 6.70
CHEMBL472 GLYBURIDE 4.0 1 5.64
CHEMBL5268127 1 4.86
CHEMBL165 RESVERATROL 3.0 1 4.11
CHEMBL2071526 ALTISSIMACOUMARIN D 1 -
CHEMBL307341 AURAPTENE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL595 PIOGLITAZONE EC50 = 200.0 nM 6.70
CHEMBL472 GLYBURIDE EC50 = 2300.0 nM 5.64
CHEMBL5268127 EC50 = 13800.0 nM 4.86
CHEMBL165 RESVERATROL EC50 = 77100.0 nM 4.11
CHEMBL2071526 ALTISSIMACOUMARIN D EC50 > 30000.0 nM -
CHEMBL307341 AURAPTENE EC50 > 30000.0 nM -