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Assay Detail

CHEMBL5230436

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant ACE2 at 50 uM using (7Mca-Y-V-A- D -A-P- K(Knp) as a flurogenic substrate measured after 10 mins by fluorescence based analysis
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Angiotensin-converting enzyme 2 (CHEMBL3736)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Inhibitors of SARS-CoV-2 Entry: Current and Future Opportunities.
Xiu S, Dick A, Ju H, Mirzaie S, Abdi F, Cocklin S, Zhan P, Liu X.
J Med Chem (2020) 63 : 12256 -12274
PubMed 32539378 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 6.91 9.36

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5279656 1 9.36
CHEMBL5281591 1 9.00
CHEMBL5277567 1 7.00
CHEMBL5270233 1 6.70
CHEMBL1535 HYDROXYCHLOROQUINE 4.0 1 6.14
CHEMBL76 CHLOROQUINE 4.0 1 5.95
CHEMBL398940 1 4.24

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5279656 IC50 = 0.44 nM 9.36
CHEMBL5281591 IC50 = 1.0 nM 9.00
CHEMBL5277567 IC50 = 100.0 nM 7.00
CHEMBL5270233 IC50 = 200.0 nM 6.70
CHEMBL1535 HYDROXYCHLOROQUINE IC50 = 720.0 nM 6.14
CHEMBL76 CHLOROQUINE IC50 = 1130.0 nM 5.95
CHEMBL398940 IC50 = 57000.0 nM 4.24