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Assay Detail

CHEMBL5232751

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human DHFR
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL202)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Chemotherapeutics for <i>Toxoplasma gondii</i>: Molecular Biotargets, Binding Modes, and Structure-Activity Relationship Investigations.
Wu RZ, Zhou HY, Song JF, Xia QH, Hu W, Mou XD, Li X.
J Med Chem (2021) 64 : 17627 -17655
PubMed 34894691 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 7.34 9.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL119 TRIMETREXATE 4.0 1 9.38
CHEMBL32113 1 7.58
CHEMBL32708 1 5.07

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL119 TRIMETREXATE IC50 = 0.42 nM 9.38
CHEMBL32113 IC50 = 26.0 nM 7.58
CHEMBL32708 IC50 = 8500.0 nM 5.07