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Assay Detail

CHEMBL5236876

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST-tagged TTBK1 (1 to 421) (unknown origin) using DNA Topoisomerase 2-alpha-Thr1342 as substrate incubated for 15 mins followed by ATP addition and further incubated for 120 mins by TR-FRET assay
32
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tau-tubulin kinase 1 (CHEMBL1926492)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Potent and Brain-Penetrant Tau Tubulin Kinase 1 (TTBK1) Inhibitors that Lower Tau Phosphorylation In Vivo.
Halkina T, Henderson JL, Lin EY, Himmelbauer MK, Jones JH, Nevalainen M, Feng J, King K, Rooney M, Johnson JL, Marcotte DJ, Chodaparambil JV, Kumar PR, Patterson TA, Murugan P, Schuman E, Wong L, Hesson T, Lamore S, Bao C, Calhoun M, Certo H, Amaral B, Dillon GM, Gilfillan R, de Turiso FG.
J Med Chem (2021) 64 : 6358 -6380
PubMed 33944571 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 32 7.13 8.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5268795 1 8.64
CHEMBL5283456 1 8.64
CHEMBL5280210 1 8.57
CHEMBL5272364 1 8.55
CHEMBL5279863 1 8.11
CHEMBL5282072 1 8.01
CHEMBL5268080 1 8.00
CHEMBL5271137 1 7.75
CHEMBL5273263 1 7.75
CHEMBL5282902 1 7.70
CHEMBL5290581 1 7.50
CHEMBL5274162 1 7.43
CHEMBL5280803 1 7.41
CHEMBL5288487 1 7.24
CHEMBL5278744 1 7.22
CHEMBL5274528 1 7.07
CHEMBL5290252 2 6.91
CHEMBL5279159 1 6.73
CHEMBL2334586 1 6.70
CHEMBL5275588 1 6.45
CHEMBL5272862 1 6.38
CHEMBL5181163 1 5.99
CHEMBL5283413 1 5.71
CHEMBL5193906 1 5.60
CHEMBL127907 1 5.34
CHEMBL5286028 1 4.77
CHEMBL5281273 1 -
CHEMBL5269486 1 -
CHEMBL5282564 1 -
CHEMBL5287032 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5268795 IC50 = 2.3 nM 8.64
CHEMBL5283456 IC50 = 2.3 nM 8.64
CHEMBL5280210 IC50 = 2.7 nM 8.57
CHEMBL5272364 IC50 = 2.8 nM 8.55
CHEMBL5279863 IC50 = 7.8 nM 8.11
CHEMBL5282072 IC50 = 9.8 nM 8.01
CHEMBL5268080 IC50 = 10.0 nM 8.00
CHEMBL5273263 IC50 = 18.0 nM 7.75
CHEMBL5271137 IC50 = 18.0 nM 7.75
CHEMBL5282902 IC50 = 20.0 nM 7.70
CHEMBL5290581 IC50 = 32.0 nM 7.50
CHEMBL5274162 IC50 = 37.0 nM 7.43
CHEMBL5280803 IC50 = 39.0 nM 7.41
CHEMBL5288487 IC50 = 57.0 nM 7.24
CHEMBL5278744 IC50 = 60.0 nM 7.22
CHEMBL5274528 IC50 = 86.0 nM 7.07
CHEMBL5290252 IC50 = 124.0 nM 6.91
CHEMBL5279159 IC50 = 185.0 nM 6.73
CHEMBL2334586 IC50 = 199.0 nM 6.70
CHEMBL5275588 IC50 = 352.0 nM 6.45
CHEMBL5290252 IC50 = 352.0 nM 6.45
CHEMBL5272862 IC50 = 414.0 nM 6.38
CHEMBL5181163 IC50 = 1022.0 nM 5.99
CHEMBL5283413 IC50 = 1942.0 nM 5.71
CHEMBL5193906 IC50 = 2510.0 nM 5.60
CHEMBL127907 IC50 = 4610.0 nM 5.34
CHEMBL5286028 IC50 = 16850.0 nM 4.77
CHEMBL5281273 IC50 > 100000.0 nM -
CHEMBL5269486 IC50 > 100000.0 nM -
CHEMBL5282564 IC50 > 100000.0 nM -
CHEMBL5287032 IC50 > 100000.0 nM -
CHEMBL5287975 IC50 > 100000.0 nM -