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Assay Detail

CHEMBL5240451

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inverse agonist activity at human PPARgamma in human RT112/84 transfected with NLuc-fused FABP4 assessed as reduction in PPARgamma transactivation incubated for 6 to 18 hrs by Nano-Glo luciferase assay
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type RT112/84
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor gamma (CHEMBL235)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and characterization of orally bioavailable 4-chloro-6-fluoroisophthalamides as covalent PPARG inverse-agonists.
Orsi DL, Ferrara SJ, Siegel S, Friberg A, Bouché L, Pook E, Lienau P, Bluck JP, Lemke CT, Akcay G, Stellfeld T, Meyer H, Pütter V, Holton SJ, Korr D, Jerchel-Furau I, Pantelidou C, Strathdee CA, Meyerson M, Eis K, Goldstein JT.
Bioorg Med Chem (2023) 78 : 117130 -117130
PubMed 36542958 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 7.22 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5289955 1 8.70
CHEMBL5267263 1 8.38
CHEMBL5273393 1 8.32
CHEMBL5282503 1 8.30
CHEMBL5281842 1 8.18
CHEMBL5268912 1 8.13
CHEMBL5271038 1 8.08
CHEMBL5272194 1 7.85
CHEMBL5286386 1 7.82
CHEMBL5285688 1 7.42
CHEMBL5291018 1 7.27
CHEMBL5277957 1 7.04
CHEMBL5287868 1 7.03
CHEMBL5283176 1 6.74
CHEMBL5288593 1 5.98
CHEMBL5278668 1 5.95
CHEMBL5282181 1 5.66
CHEMBL5287866 1 5.28
CHEMBL5273751 1 5.13
CHEMBL5282748 1 -
CHEMBL5287514 1 -
CHEMBL5281604 1 -
CHEMBL5272116 1 -
CHEMBL5273188 1 -
CHEMBL5286808 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5289955 IC50 = 2.0 nM 8.70
CHEMBL5267263 IC50 = 4.2 nM 8.38
CHEMBL5273393 IC50 = 4.8 nM 8.32
CHEMBL5282503 IC50 = 5.0 nM 8.30
CHEMBL5281842 IC50 = 6.6 nM 8.18
CHEMBL5268912 IC50 = 7.35 nM 8.13
CHEMBL5271038 IC50 = 8.3 nM 8.08
CHEMBL5272194 IC50 = 14.0 nM 7.85
CHEMBL5286386 IC50 = 15.0 nM 7.82
CHEMBL5285688 IC50 = 38.0 nM 7.42
CHEMBL5291018 IC50 = 54.0 nM 7.27
CHEMBL5277957 IC50 = 91.0 nM 7.04
CHEMBL5287868 IC50 = 94.0 nM 7.03
CHEMBL5283176 IC50 = 183.0 nM 6.74
CHEMBL5288593 IC50 = 1040.0 nM 5.98
CHEMBL5278668 IC50 = 1120.0 nM 5.95
CHEMBL5282181 IC50 = 2200.0 nM 5.66
CHEMBL5287866 IC50 = 5250.0 nM 5.28
CHEMBL5273751 IC50 = 7400.0 nM 5.13
CHEMBL5282748 IC50 > 5000.0 nM -
CHEMBL5287514 IC50 > 5000.0 nM -
CHEMBL5281604 IC50 > 5000.0 nM -
CHEMBL5272116 IC50 > 5000.0 nM -
CHEMBL5273188 IC50 > 5000.0 nM -
CHEMBL5286808 IC50 > 5000.0 nM -