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Assay Detail

CHEMBL5252014

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of tau 4R1N phosphorylation (unknown origin) at S396 residue transfected in human U2OS cells coexpressing GFP-tagged beta catenin incubated overnight by high content imaging assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type U2OS
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Microtubule-associated protein tau (CHEMBL1293224)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Structure-Activity Relationships, and In Vivo Evaluation of Potent and Brain-Penetrant Imidazo[1,2-<i>b</i>]pyridazines as Glycogen Synthase Kinase-3β (GSK-3β) Inhibitors.
Hartz RA, Ahuja VT, Sivaprakasam P, Xiao H, Krause CM, Clarke WJ, Kish K, Lewis H, Szapiel N, Ravirala R, Mutalik S, Nakmode D, Shah D, Burton CR, Macor JE, Dubowchik GM.
J Med Chem (2023) 66 : 4231 -4252
PubMed 36950863 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 7.17 8.04

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5266553 1 8.04
CHEMBL5270555 1 7.52
CHEMBL5282319 1 7.44
CHEMBL5285422 1 7.39
CHEMBL5270781 1 7.24
CHEMBL5273327 1 7.24
CHEMBL5274596 1 7.22
CHEMBL5290040 1 7.08
CHEMBL5316226 1 7.07
CHEMBL5282376 1 6.92
CHEMBL5286737 1 6.89
CHEMBL5286669 1 6.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5266553 IC50 = 9.2 nM 8.04
CHEMBL5270555 IC50 = 30.0 nM 7.52
CHEMBL5282319 IC50 = 36.0 nM 7.44
CHEMBL5285422 IC50 = 41.0 nM 7.39
CHEMBL5270781 IC50 = 58.0 nM 7.24
CHEMBL5273327 IC50 = 58.0 nM 7.24
CHEMBL5274596 IC50 = 60.0 nM 7.22
CHEMBL5290040 IC50 = 83.0 nM 7.08
CHEMBL5316226 IC50 = 86.0 nM 7.07
CHEMBL5282376 IC50 = 120.0 nM 6.92
CHEMBL5286737 IC50 = 130.0 nM 6.89
CHEMBL5286669 IC50 = 910.0 nM 6.04