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Assay Detail

CHEMBL5254890

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HIV-1 protease using Abz-NF-6 as substrate assessed as inhibition constant measured every 2 mins for 20 mins by fluorescence based ELISA analysis
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Human immunodeficiency virus 1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Human immunodeficiency virus type 1 protease (CHEMBL243)
Type SINGLE PROTEIN
Organism Human immunodeficiency virus 1

Publication

Evaluation of darunavir-derived HIV-1 protease inhibitors incorporating P2' amide-derivatives: Synthesis, biological evaluation and structural studies.
Ghosh AK, Shahabi D, Kipfmiller M, Ghosh AK, Johnson M, Wang YF, Agniswamy J, Amano M, Weber IT, Mitsuya H.
Bioorg Med Chem Lett (2023) 83 : 129168 -129168
PubMed 36738797 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 8.86 10.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5274743 1 10.89
CHEMBL1323 DARUNAVIR 4.0 1 10.80
CHEMBL5287711 1 10.04
CHEMBL5271083 1 9.52
CHEMBL5288746 1 9.41
CHEMBL5284706 1 9.35
CHEMBL5268648 1 6.75
CHEMBL5281851 1 6.51
CHEMBL5278603 1 6.47
CHEMBL5271223 1 -
CHEMBL5287201 1 -
CHEMBL5291125 1 -
CHEMBL5280093 1 -
CHEMBL5267260 1 -
CHEMBL5266228 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5274743 Ki = 0.0129 nM 10.89
CHEMBL1323 DARUNAVIR Ki = 0.016 nM 10.80
CHEMBL5287711 Ki = 0.0921 nM 10.04
CHEMBL5271083 Ki = 0.302 nM 9.52
CHEMBL5288746 Ki = 0.388 nM 9.41
CHEMBL5284706 Ki = 0.445 nM 9.35
CHEMBL5268648 Ki = 177.8 nM 6.75
CHEMBL5281851 Ki = 310.37 nM 6.51
CHEMBL5278603 Ki = 337.3 nM 6.47
CHEMBL5271223 Ki = 0.0089 nM -
CHEMBL5287201 Ki = 0.0044 nM -
CHEMBL5291125 Ki = 0.0006 nM -
CHEMBL5280093 Ki = 0.0091 nM -
CHEMBL5267260 Ki = 0.0053 nM -
CHEMBL5266228 Ki = 0.0015 nM -