Assay Detail
Binding
CHEMBL5256364
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Inhibition of Shh-induced hedgehog signalling pathway in mouse NIH/3T3 cells expressing Gli-Luc reporter gene assessed as reduction in Gli1 transcription activity incubated for 24 hrs in presence of mouse shh stimulation by dual luciferase Assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Cell Type | NIH/3T3 |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Hydroxylated cyclopamine analogues from Veratrum californicum and their hedgehog pathway inhibiting activity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 6.36 | 6.82 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5281898 | — | — | 1 | 6.82 |
| CHEMBL254129 | CYCLOPAMINE | — | 1 | 6.57 |
| CHEMBL5283898 | — | — | 1 | 5.68 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5281898 | — | IC50 | = | 150.0 | nM | 6.82 |
| CHEMBL254129 | CYCLOPAMINE | IC50 | = | 270.0 | nM | 6.57 |
| CHEMBL5283898 | — | IC50 | = | 2080.0 | nM | 5.68 |