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Assay Detail

CHEMBL5316641

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-Epibatidine from alpha4beta2 nAChR (unknown origin) expressed in HEK293 cell membrane assessed as inhibition constant by beta counter method
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Subcellular Membrane
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907589)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Subnanomolar Affinity and Selective Antagonism at α7 Nicotinic Receptor by Combined Modifications of 2-Triethylammonium Ethyl Ether of 4-Stilbenol (MG624).
Bavo F, Pallavicini M, Pucci S, Appiani R, Giraudo A, Oh H, Kneisley DL, Eaton B, Lucero L, Gotti C, Clementi F, Whiteaker P, Bolchi C.
J Med Chem (2023) 66 : 306 -332
PubMed 36526469 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 33 5.06 5.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4287656 1 5.92
CHEMBL5423336 1 5.75
CHEMBL5396579 1 5.35
CHEMBL5393773 1 5.29
CHEMBL5403618 1 5.14
CHEMBL5415255 1 5.12
CHEMBL5407945 1 5.01
CHEMBL5418191 1 5.00
CHEMBL5415576 1 4.98
CHEMBL5435415 1 4.93
CHEMBL5396724 1 4.72
CHEMBL5415788 1 4.71
CHEMBL5406306 1 4.66
CHEMBL5436520 1 4.64
CHEMBL5435510 1 4.62
CHEMBL5416363 1 -
CHEMBL5412463 1 -
CHEMBL5396300 1 -
CHEMBL5425014 1 -
CHEMBL5428774 1 -
CHEMBL1257065 STILONIUM IODIDE 2.0 1 -
CHEMBL5395819 1 -
CHEMBL5400651 1 -
CHEMBL5417751 1 -
CHEMBL5409285 1 -
CHEMBL5433741 1 -
CHEMBL5416962 1 -
CHEMBL5408237 1 -
CHEMBL5432840 1 -
CHEMBL5415738 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4287656 Ki = 1200.0 nM 5.92
CHEMBL5423336 Ki = 1800.0 nM 5.75
CHEMBL5396579 Ki = 4500.0 nM 5.35
CHEMBL5393773 Ki = 5100.0 nM 5.29
CHEMBL5403618 Ki = 7240.0 nM 5.14
CHEMBL5415255 Ki = 7500.0 nM 5.12
CHEMBL5407945 Ki = 9820.0 nM 5.01
CHEMBL5418191 Ki = 10100.0 nM 5.00
CHEMBL5415576 Ki = 10400.0 nM 4.98
CHEMBL5435415 Ki = 11800.0 nM 4.93
CHEMBL5396724 Ki = 19000.0 nM 4.72
CHEMBL5415788 Ki = 19400.0 nM 4.71
CHEMBL5406306 Ki = 21800.0 nM 4.66
CHEMBL5436520 Ki = 23000.0 nM 4.64
CHEMBL5435510 Ki = 24000.0 nM 4.62
CHEMBL5178473 Ki = 9300.0 nM -
CHEMBL5399641 Ki - -
CHEMBL5415482 Ki - -
CHEMBL5416363 Ki - -
CHEMBL5412463 Ki - -
CHEMBL5396300 Ki - -
CHEMBL5425014 Ki - -
CHEMBL5428774 Ki - -
CHEMBL1257065 STILONIUM IODIDE Ki = 5700.0 nM -
CHEMBL5395819 Ki - -
CHEMBL5400651 Ki - -
CHEMBL5417751 Ki - -
CHEMBL5409285 Ki - -
CHEMBL5433741 Ki - -
CHEMBL5416962 Ki - -
CHEMBL5408237 Ki - -
CHEMBL5432840 Ki - -
CHEMBL5415738 Ki - -