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Assay Detail

CHEMBL5316663

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of CD73 in human plasma incubated for 15 mins in presence of 15N5-AMP by LC/MS analysis
22
Total Activities
21
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Plasma
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5'-nucleotidase (CHEMBL5957)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Series of Potent, Selective, and Orally Bioavailable Nucleoside Inhibitors of CD73 That Demonstrates <i>In Vivo</i> Antitumor Activity.
Li J, Chen L, Billedeau RJ, Stanton TF, Chiang JTP, Lee CC, Li W, Steggerda S, Emberley E, Gross M, Bhupathi D, Che X, Chen J, Dang R, Huang T, Ma Y, MacKinnon A, Makkouk A, Marguier G, Neou S, Sotirovska N, Spurlock S, Zhang J, Zhang W, van Zandt M, Yuan L, Savoy J, Parlati F, Sjogren EB.
J Med Chem (2023) 66 : 345 -370
PubMed 36529947 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 7.99 9.59
IC90 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5439071 1 9.59
CHEMBL5423436 2 9.42
CHEMBL5404625 1 9.42
CHEMBL5403339 1 8.82
CHEMBL5398515 1 8.80
CHEMBL5398484 1 8.55
CHEMBL5415675 1 8.27
CHEMBL5393743 1 8.25
CHEMBL5435185 1 8.19
CHEMBL5404674 1 8.14
CHEMBL5417110 1 7.94
CHEMBL5415259 1 7.81
CHEMBL5421027 1 7.81
CHEMBL5404795 1 7.80
CHEMBL5416552 1 7.39
CHEMBL5423764 1 7.30
CHEMBL5398262 1 6.87
CHEMBL5428426 1 6.80
CHEMBL5409033 1 6.68
CHEMBL5410317 1 6.04
CHEMBL5419527 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5439071 IC50 = 0.26 nM 9.59
CHEMBL5423436 IC50 = 0.38 nM 9.42
CHEMBL5404625 IC50 = 0.38 nM 9.42
CHEMBL5403339 IC50 = 1.5 nM 8.82
CHEMBL5398515 IC50 = 1.6 nM 8.80
CHEMBL5398484 IC50 = 2.8 nM 8.55
CHEMBL5415675 IC50 = 5.4 nM 8.27
CHEMBL5393743 IC50 = 5.6 nM 8.25
CHEMBL5435185 IC50 = 6.5 nM 8.19
CHEMBL5404674 IC50 = 7.2 nM 8.14
CHEMBL5417110 IC50 = 11.5 nM 7.94
CHEMBL5415259 IC50 = 15.6 nM 7.81
CHEMBL5421027 IC50 = 15.5 nM 7.81
CHEMBL5404795 IC50 = 16.0 nM 7.80
CHEMBL5416552 IC50 = 41.0 nM 7.39
CHEMBL5423764 IC50 = 50.0 nM 7.30
CHEMBL5398262 IC50 = 136.0 nM 6.87
CHEMBL5428426 IC50 = 160.0 nM 6.80
CHEMBL5409033 IC50 = 208.0 nM 6.68
CHEMBL5410317 IC50 = 917.0 nM 6.04
CHEMBL5419527 IC50 > 100.0 nM -
CHEMBL5423436 IC90 = 3.0 nM -