Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5316668

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of C-terminal 6His-tagged recombinant mouse CD73 (29 to 549 residues) expressed in CHO cells incubated for 1 hr by malachite green assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5'-nucleotidase (CHEMBL4680034)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Discovery of a Series of Potent, Selective, and Orally Bioavailable Nucleoside Inhibitors of CD73 That Demonstrates <i>In Vivo</i> Antitumor Activity.
Li J, Chen L, Billedeau RJ, Stanton TF, Chiang JTP, Lee CC, Li W, Steggerda S, Emberley E, Gross M, Bhupathi D, Che X, Chen J, Dang R, Huang T, Ma Y, MacKinnon A, Makkouk A, Marguier G, Neou S, Sotirovska N, Spurlock S, Zhang J, Zhang W, van Zandt M, Yuan L, Savoy J, Parlati F, Sjogren EB.
J Med Chem (2023) 66 : 345 -370
PubMed 36529947 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 8.52 8.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5423436 1 8.89
CHEMBL5393743 1 8.14

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5423436 IC50 = 1.3 nM 8.89
CHEMBL5393743 IC50 = 7.3 nM 8.14