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Assay Detail

CHEMBL5316781

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of Cy5 modified rapamycin from GST tagged FKBP12 (1 to 108 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) incubated for 6 hrs by fluorescence plate reader analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of RMC-5552, a Selective Bi-Steric Inhibitor of mTORC1, for the Treatment of mTORC1-Activated Tumors.
Burnett GL, Yang YC, Aggen JB, Pitzen J, Gliedt MK, Semko CM, Marquez A, Evans JW, Wang G, Won WS, Tomlinson ACA, Kiss G, Tzitzilonis C, Thottumkara AP, Cregg J, Mellem KT, Choi JS, Lee JC, Zhao Y, Lee BJ, Meyerowitz JG, Knox JE, Jiang J, Wang Z, Wildes D, Wang Z, Singh M, Smith JAM, Gill AL.
J Med Chem (2023) 66 : 149 -169
PubMed 36533617 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 3 7.80 9.36

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL413 SIROLIMUS 4.0 1 9.36
CHEMBL5418335 1 7.88
CHEMBL5431157 1 6.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL413 SIROLIMUS Kd = 0.44 nM 9.36
CHEMBL5418335 Kd = 13.3 nM 7.88
CHEMBL5431157 Kd = 701.0 nM 6.15