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Assay Detail

CHEMBL5317801

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Clostridioides difficile-specific DNA adenine methyltransferase PDB 7RFL using 5'-CGA TTC AAA AAG TCC CAA G-3' and 3'-GCT AAG TTT TTC AGG GTT C-5' as substrate and SAM as cosubstrate preincubated for 5 mins followed by substrate and cosubstrate addition and measured after 3 mins by MTase-Glo assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Clostridioides difficile
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Systematic Design of Adenosine Analogs as Inhibitors of a <i>Clostridioides difficile-</i>Specific DNA Adenine Methyltransferase Required for Normal Sporulation and Persistence.
Zhou J, Horton JR, Menna M, Fiorentino F, Ren R, Yu D, Hajian T, Vedadi M, Mazzoccanti G, Ciogli A, Weinhold E, Hüben M, Blumenthal RM, Zhang X, Mai A, Rotili D, Cheng X.
J Med Chem (2023) 66 : 934 -950
PubMed 36581322 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 5.33 5.33

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3087498 1 5.33

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3087498 IC50 = 4700.0 nM 5.33