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Assay Detail

CHEMBL5317827

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MCT1 transport activity in human A549 cells using 3-BP as substrate assessed as reduction in intracellular 3-BP level by measuring increase in cell viability incubated for 72 hrs by MTT assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type A549
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Monocarboxylate transporter 1 (CHEMBL4360)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Indole Derivatives as New Structural Class of Potent and Antiproliferative Inhibitors of Monocarboxylate Transporter 1 (MCT1; SLC16A1).
Puri S, Stefan K, Khan SL, Pahnke J, Stefan SM, Juvale K.
J Med Chem (2023) 66 : 657 -676
PubMed 36584238 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 6.81 7.09

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5435709 1 7.09
CHEMBL5419493 1 7.08
CHEMBL76602 alpha-CYANO-4-HYDROXYCINNAMIC ACID 1 7.06
CHEMBL5401494 1 6.98
CHEMBL5405329 1 6.98
CHEMBL5409450 1 6.96
CHEMBL5433941 1 6.96
CHEMBL5394985 1 6.96
CHEMBL5440350 1 6.95
CHEMBL5421503 1 6.87
CHEMBL5427293 1 6.71
CHEMBL5397576 1 6.71
CHEMBL5431419 1 6.70
CHEMBL5439235 1 6.67
CHEMBL5402273 1 6.54
CHEMBL5402599 1 6.50
CHEMBL5427387 1 6.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5435709 IC50 = 81.0 nM 7.09
CHEMBL5419493 IC50 = 82.3 nM 7.08
CHEMBL76602 alpha-CYANO-4-HYDROXYCINNAMIC ACID IC50 = 87.2 nM 7.06
CHEMBL5401494 IC50 = 105.0 nM 6.98
CHEMBL5405329 IC50 = 105.0 nM 6.98
CHEMBL5409450 IC50 = 109.0 nM 6.96
CHEMBL5433941 IC50 = 109.0 nM 6.96
CHEMBL5394985 IC50 = 109.0 nM 6.96
CHEMBL5440350 IC50 = 112.0 nM 6.95
CHEMBL5421503 IC50 = 135.0 nM 6.87
CHEMBL5427293 IC50 = 196.0 nM 6.71
CHEMBL5397576 IC50 = 193.0 nM 6.71
CHEMBL5431419 IC50 = 198.0 nM 6.70
CHEMBL5439235 IC50 = 214.0 nM 6.67
CHEMBL5402273 IC50 = 287.0 nM 6.54
CHEMBL5402599 IC50 = 314.0 nM 6.50
CHEMBL5427387 IC50 = 793.0 nM 6.10