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Assay Detail

CHEMBL5318375

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at rat TRPA1 channel expressed in CHO-K1 cells tagged with GCaMP6 by measuring calcium flux measured after 10 mins by FLIPR tetra analysis
39
Total Activities
37
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of <b>BAY-390</b>, a Selective CNS Penetrant Chemical Probe as Transient Receptor Potential Ankyrin 1 (TRPA1) Antagonist.
Mesch S, Walter D, Laux-Biehlmann A, Basting D, Flanagan S, Miyatake Ondozabal H, Bäurle S, Pearson C, Jenkins J, Elves P, Hess S, Coelho AM, Rotgeri A, Bothe U, Nawaz S, Zollner TM, Steinmeyer A.
J Med Chem (2023) 66 : 1583 -1600
PubMed 36622903 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 39 5.79 7.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5428371 1 7.62
CHEMBL4514203 BAY-390 1 7.20
CHEMBL5394758 1 6.93
CHEMBL5439762 1 6.92
CHEMBL5428179 1 6.89
CHEMBL5419744 1 6.70
CHEMBL5413437 1 6.21
CHEMBL5422541 1 6.19
CHEMBL5423752 1 6.17
CHEMBL5437183 3 6.07
CHEMBL5413993 1 6.02
CHEMBL5440996 1 5.92
CHEMBL5394738 1 5.89
CHEMBL5398163 1 5.86
CHEMBL5426246 1 5.64
CHEMBL5440987 1 5.62
CHEMBL5408516 1 5.42
CHEMBL5411635 1 5.42
CHEMBL5424148 1 5.28
CHEMBL5419942 1 5.20
CHEMBL5436178 1 5.12
CHEMBL5419147 1 5.00
CHEMBL5431800 1 4.94
CHEMBL5401746 1 4.89
CHEMBL5407661 1 4.82
CHEMBL5398623 1 4.80
CHEMBL5397630 1 4.68
CHEMBL5431088 1 4.60
CHEMBL5436224 1 -
CHEMBL5397294 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5428371 IC50 = 24.0 nM 7.62
CHEMBL4514203 BAY-390 IC50 = 63.0 nM 7.20
CHEMBL5394758 IC50 = 118.0 nM 6.93
CHEMBL5439762 IC50 = 120.0 nM 6.92
CHEMBL5428179 IC50 = 130.0 nM 6.89
CHEMBL5419744 IC50 = 200.0 nM 6.70
CHEMBL5413437 IC50 = 620.0 nM 6.21
CHEMBL5422541 IC50 = 650.0 nM 6.19
CHEMBL5423752 IC50 = 680.0 nM 6.17
CHEMBL5437183 IC50 = 860.0 nM 6.07
CHEMBL5413993 IC50 = 950.0 nM 6.02
CHEMBL5440996 IC50 = 1200.0 nM 5.92
CHEMBL5394738 IC50 = 1300.0 nM 5.89
CHEMBL5398163 IC50 = 1370.0 nM 5.86
CHEMBL5437183 IC50 = 1560.0 nM 5.81
CHEMBL5426246 IC50 = 2300.0 nM 5.64
CHEMBL5440987 IC50 = 2400.0 nM 5.62
CHEMBL5408516 IC50 = 3800.0 nM 5.42
CHEMBL5411635 IC50 = 3800.0 nM 5.42
CHEMBL5424148 IC50 = 5300.0 nM 5.28
CHEMBL5419942 IC50 = 6300.0 nM 5.20
CHEMBL5436178 IC50 = 7570.0 nM 5.12
CHEMBL5419147 IC50 = 10000.0 nM 5.00
CHEMBL5431800 IC50 = 11500.0 nM 4.94
CHEMBL5401746 IC50 = 13000.0 nM 4.89
CHEMBL5407661 IC50 = 15000.0 nM 4.82
CHEMBL5398623 IC50 = 16000.0 nM 4.80
CHEMBL5397630 IC50 = 21000.0 nM 4.68
CHEMBL5431088 IC50 = 25000.0 nM 4.60
CHEMBL5437183 IC50 > 25000.0 nM -
CHEMBL5394183 IC50 > 25000.0 nM -
CHEMBL5428534 IC50 > 25000.0 nM -
CHEMBL4555711 BAY-9897 IC50 > 25000.0 nM -
CHEMBL5419068 IC50 > 12500.0 nM -
CHEMBL5438649 IC50 > 12500.0 nM -
CHEMBL5436224 IC50 > 10000.0 nM -
CHEMBL5396560 IC50 > 12500.0 nM -
CHEMBL5397294 IC50 > 25000.0 nM -
CHEMBL5424431 IC50 > 25000.0 nM -