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Assay Detail

CHEMBL5318512

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal His6-tagged full-length recombinant beta-catenin (1 to 781 residues) (unknown origin) expressed in Escherichia coli BL21 DE3/N-terminal FAM tagged BCL9 HD2(350 to 375 residues) (unknown origin) protein-protein interaction assessed as inhibition constant incubated for 3 hrs by competitive fluorescence polarization assay
39
Total Activities
39
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

beta-catenin-B-cell lymphoma 9 protein complex (CHEMBL3885525)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of Novel 3-Phenylpiperidine Derivatives Targeting the β-Catenin/B-Cell Lymphoma 9 Interaction as a Single Agent and in Combination with the Anti-PD-1 Antibody for the Treatment of Colorectal Cancer.
Zhang H, Liu C, Chen Q, Shen LA, Xiao W, Li J, Wang Y, Zhu D, Zhang Q, Li J.
J Med Chem (2023) 66 : 1349 -1379
PubMed 36630177 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 39 5.55 6.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5398967 1 6.19
CHEMBL5416539 1 6.14
CHEMBL5404409 1 6.05
CHEMBL5413417 1 6.04
CHEMBL5422967 1 6.00
CHEMBL5399058 1 5.96
CHEMBL5400908 1 5.93
CHEMBL5440529 1 5.93
CHEMBL5398170 1 5.90
CHEMBL5406240 1 5.80
CHEMBL5420343 1 5.73
CHEMBL5397851 1 5.61
CHEMBL5418296 1 5.50
CHEMBL5422085 1 5.49
CHEMBL5412754 1 5.42
CHEMBL5424713 1 5.41
CHEMBL5398771 1 5.40
CHEMBL5394479 1 5.38
CHEMBL5440087 1 5.37
CHEMBL5428569 1 5.37
CHEMBL5399118 1 5.35
CHEMBL5435959 1 5.33
CHEMBL5411500 1 5.31
CHEMBL5396445 1 5.29
CHEMBL5436700 1 5.28
CHEMBL5396996 1 5.28
CHEMBL5433123 1 5.27
CHEMBL5440988 1 5.27
CHEMBL5395861 1 5.17
CHEMBL5409422 1 5.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5398967 IC50 = 650.0 nM 6.19
CHEMBL5416539 IC50 = 720.0 nM 6.14
CHEMBL5404409 IC50 = 890.0 nM 6.05
CHEMBL5413417 IC50 = 910.0 nM 6.04
CHEMBL5422967 IC50 = 1000.0 nM 6.00
CHEMBL5399058 IC50 = 1100.0 nM 5.96
CHEMBL5400908 IC50 = 1180.0 nM 5.93
CHEMBL5440529 IC50 = 1180.0 nM 5.93
CHEMBL5398170 IC50 = 1270.0 nM 5.90
CHEMBL5406240 IC50 = 1580.0 nM 5.80
CHEMBL5420343 IC50 = 1860.0 nM 5.73
CHEMBL5397851 IC50 = 2470.0 nM 5.61
CHEMBL5418296 IC50 = 3140.0 nM 5.50
CHEMBL5422085 IC50 = 3270.0 nM 5.49
CHEMBL5412754 IC50 = 3760.0 nM 5.42
CHEMBL5424713 IC50 = 3900.0 nM 5.41
CHEMBL5398771 IC50 = 4000.0 nM 5.40
CHEMBL5394479 IC50 = 4200.0 nM 5.38
CHEMBL5428569 IC50 = 4270.0 nM 5.37
CHEMBL5440087 IC50 = 4320.0 nM 5.37
CHEMBL5399118 IC50 = 4470.0 nM 5.35
CHEMBL5435959 IC50 = 4630.0 nM 5.33
CHEMBL5411500 IC50 = 4860.0 nM 5.31
CHEMBL5396445 IC50 = 5190.0 nM 5.29
CHEMBL5436700 IC50 = 5210.0 nM 5.28
CHEMBL5396996 IC50 = 5270.0 nM 5.28
CHEMBL5433123 IC50 = 5390.0 nM 5.27
CHEMBL5440988 IC50 = 5390.0 nM 5.27
CHEMBL5409422 IC50 = 6700.0 nM 5.17
CHEMBL5395861 IC50 = 6800.0 nM 5.17
CHEMBL5431783 IC50 = 8300.0 nM 5.08
CHEMBL5395984 IC50 = 8620.0 nM 5.06
CHEMBL5433291 IC50 > 10000.0 nM -
CHEMBL5416247 IC50 > 10000.0 nM -
CHEMBL5436831 IC50 > 10000.0 nM -
CHEMBL5438849 IC50 > 10000.0 nM -
CHEMBL5440543 IC50 > 10000.0 nM -
CHEMBL5405503 IC50 > 10000.0 nM -
CHEMBL5406679 IC50 > 10000.0 nM -