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Assay Detail

CHEMBL5318513

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of beta-catenin/BCL9 protein-protein interaction in Wnt-dependent human HCT-116 cells assessed as reduction in Axin2 expression level by qRT-PCR assay
40
Total Activities
40
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HCT-116
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

beta-catenin-B-cell lymphoma 9 protein complex (CHEMBL3885525)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of Novel 3-Phenylpiperidine Derivatives Targeting the β-Catenin/B-Cell Lymphoma 9 Interaction as a Single Agent and in Combination with the Anti-PD-1 Antibody for the Treatment of Colorectal Cancer.
Zhang H, Liu C, Chen Q, Shen LA, Xiao W, Li J, Wang Y, Zhu D, Zhang Q, Li J.
J Med Chem (2023) 66 : 1349 -1379
PubMed 36630177 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 40 5.73 6.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5398967 1 6.16
CHEMBL5416539 1 6.09
CHEMBL5413417 1 6.03
CHEMBL5440529 1 5.96
CHEMBL5398170 1 5.95
CHEMBL5422967 1 5.92
CHEMBL5418296 1 5.88
CHEMBL5397851 1 5.88
CHEMBL5400908 1 5.87
CHEMBL5399058 1 5.86
CHEMBL5420343 1 5.83
CHEMBL5406240 1 5.65
CHEMBL2312139 1 5.57
CHEMBL5404409 1 5.53
CHEMBL5422085 1 5.45
CHEMBL5412754 1 5.00
CHEMBL5433123 1 4.84
CHEMBL5399118 1 -
CHEMBL5416247 1 -
CHEMBL5431783 1 -
CHEMBL5440543 1 -
CHEMBL5398771 1 -
CHEMBL5424713 1 -
CHEMBL5436700 1 -
CHEMBL5405503 1 -
CHEMBL5409422 1 -
CHEMBL5394479 1 -
CHEMBL5396996 1 -
CHEMBL5395861 1 -
CHEMBL5440087 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5398967 IC50 = 690.0 nM 6.16
CHEMBL5416539 IC50 = 820.0 nM 6.09
CHEMBL5413417 IC50 = 940.0 nM 6.03
CHEMBL5440529 IC50 = 1100.0 nM 5.96
CHEMBL5398170 IC50 = 1130.0 nM 5.95
CHEMBL5422967 IC50 = 1190.0 nM 5.92
CHEMBL5418296 IC50 = 1310.0 nM 5.88
CHEMBL5397851 IC50 = 1330.0 nM 5.88
CHEMBL5400908 IC50 = 1360.0 nM 5.87
CHEMBL5399058 IC50 = 1380.0 nM 5.86
CHEMBL5420343 IC50 = 1490.0 nM 5.83
CHEMBL5406240 IC50 = 2260.0 nM 5.65
CHEMBL2312139 IC50 = 2690.0 nM 5.57
CHEMBL5404409 IC50 = 2930.0 nM 5.53
CHEMBL5422085 IC50 = 3580.0 nM 5.45
CHEMBL5412754 IC50 = 10000.0 nM 5.00
CHEMBL5433123 IC50 = 14560.0 nM 4.84
CHEMBL5435959 IC50 - -
CHEMBL5428569 IC50 - -
CHEMBL5433291 IC50 - -
CHEMBL5440988 IC50 - -
CHEMBL5395984 IC50 - -
CHEMBL5411500 IC50 - -
CHEMBL5436831 IC50 - -
CHEMBL5438849 IC50 - -
CHEMBL5406679 IC50 - -
CHEMBL5396445 IC50 - -
CHEMBL5440087 IC50 - -
CHEMBL5395861 IC50 - -
CHEMBL5396996 IC50 - -
CHEMBL5394479 IC50 - -
CHEMBL5409422 IC50 - -
CHEMBL5405503 IC50 - -
CHEMBL5436700 IC50 - -
CHEMBL5424713 IC50 > 10000.0 nM -
CHEMBL5398771 IC50 - -
CHEMBL5440543 IC50 - -
CHEMBL5431783 IC50 - -
CHEMBL5416247 IC50 - -
CHEMBL5399118 IC50 - -