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Assay Detail

CHEMBL5319643

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant C-terminal 6His-tagged human BMPR2 (188 to 517 residues) expressed in Escherichia coli Rosetta assessed as apparent inhibition constant using ATP as substrate incubated for 30 mins by Morrison plot analysis
26
Total Activities
26
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bone morphogenetic protein receptor type-2 (CHEMBL5467)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Highly Potent and BMPR2-Selective Kinase Inhibitors Using DNA-Encoded Chemical Library Screening.
Modukuri RK, Monsivais D, Li F, Palaniappan M, Bohren KM, Tan Z, Ku AF, Wang Y, Madasu C, Li JY, Tang S, Miklossy G, Palmer SS, Young DW, Matzuk MM.
J Med Chem (2023) 66 : 2143 -2160
PubMed 36719862 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 24 7.26 8.30
Inhibition 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5404016 1 8.30
CHEMBL5404269 1 8.21
CHEMBL5422418 1 8.15
CHEMBL5434353 1 8.10
CHEMBL5420022 1 7.80
CHEMBL5437902 1 7.75
CHEMBL5398151 1 7.69
CHEMBL5436042 1 7.66
CHEMBL5428614 1 7.41
CHEMBL5402879 1 7.40
CHEMBL5421520 1 7.06
CHEMBL5409160 1 6.93
CHEMBL5408480 1 6.66
CHEMBL5397482 1 6.66
CHEMBL5399442 1 6.63
CHEMBL5400260 1 6.60
CHEMBL5438139 1 6.51
CHEMBL5411422 1 6.37
CHEMBL5434246 1 6.03
CHEMBL5420069 1 -
CHEMBL5435399 1 -
CHEMBL5433406 1 -
CHEMBL5411287 1 -
CHEMBL5420892 1 -
CHEMBL5438355 1 -
CHEMBL5417301 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5404016 Ki = 5.0 nM 8.30
CHEMBL5404269 Ki = 6.2 nM 8.21
CHEMBL5422418 Ki = 7.0 nM 8.15
CHEMBL5434353 Ki = 8.0 nM 8.10
CHEMBL5420022 Ki = 16.0 nM 7.80
CHEMBL5437902 Ki = 18.0 nM 7.75
CHEMBL5398151 Ki = 20.6 nM 7.69
CHEMBL5436042 Ki = 22.0 nM 7.66
CHEMBL5428614 Ki = 39.0 nM 7.41
CHEMBL5402879 Ki = 40.0 nM 7.40
CHEMBL5421520 Ki = 88.0 nM 7.06
CHEMBL5409160 Ki = 118.0 nM 6.93
CHEMBL5408480 Ki = 220.0 nM 6.66
CHEMBL5397482 Ki = 220.0 nM 6.66
CHEMBL5399442 Ki = 236.0 nM 6.63
CHEMBL5400260 Ki = 250.0 nM 6.60
CHEMBL5438139 Ki = 310.0 nM 6.51
CHEMBL5411422 Ki = 430.0 nM 6.37
CHEMBL5434246 Ki = 930.0 nM 6.03
CHEMBL5420069 Inhibition - % -
CHEMBL5435399 Ki > 1000.0 nM -
CHEMBL5433406 Ki > 1000.0 nM -
CHEMBL5411287 Inhibition - % -
CHEMBL5420892 Ki > 1000.0 nM -
CHEMBL5438355 Ki > 200.0 nM -
CHEMBL5417301 Ki > 200.0 nM -