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Assay Detail

CHEMBL5328164

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human mGluR2 expressed in cell membrane incubated for 1 hr in presence of tritium labeled [3H]-7-((2,5-dioxopyrrolidin-1-yl)methyl)-4-(4-fluorophenyl)quinoline-2-carboxamide by competitive radioligand binding assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Metabotropic glutamate receptor 2 (CHEMBL5137)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of [<sup>11</sup>C]MK-8056: A Selective PET Imaging Agent for the Study of mGluR<sub>2</sub> Negative Allosteric Modulators.
Perkins JJ, McQuade P, Bungard CJ, Diamond TL, Gantert LT, Gotter AL, Hanney B, Hills ID, Hurzy DM, Joshi A, Kern JT, Schlegel KS, Manikowski JJ, Meng Z, O'Brien JA, Roecker AJ, Smith SM, Uslaner JM, Hostetler E, Meissner RS.
ACS Med Chem Lett (2023) 14 : 986 -992
PubMed 37465306 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 23 8.71 9.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3955188 1 9.72
CHEMBL5405921 1 9.49
CHEMBL3945590 1 9.34
CHEMBL5409898 1 9.15
CHEMBL5418895 1 9.13
CHEMBL5429000 1 9.11
CHEMBL5394450 1 9.10
CHEMBL5435246 1 9.02
CHEMBL5429261 1 9.00
CHEMBL5436775 1 8.96
CHEMBL3979547 1 8.96
CHEMBL3950572 1 8.85
CHEMBL5405655 1 8.82
CHEMBL5410262 1 8.77
CHEMBL3892073 1 8.74
CHEMBL3895570 1 8.59
CHEMBL5429002 1 8.35
CHEMBL3910265 1 8.17
CHEMBL5425858 1 8.15
CHEMBL5399050 1 7.89
CHEMBL3902752 1 7.82
CHEMBL3981623 1 7.72
CHEMBL3926904 1 7.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3955188 Ki = 0.19 nM 9.72
CHEMBL5405921 Ki = 0.32 nM 9.49
CHEMBL3945590 Ki = 0.46 nM 9.34
CHEMBL5409898 Ki = 0.7 nM 9.15
CHEMBL5418895 Ki = 0.74 nM 9.13
CHEMBL5429000 Ki = 0.78 nM 9.11
CHEMBL5394450 Ki = 0.8 nM 9.10
CHEMBL5435246 Ki = 0.96 nM 9.02
CHEMBL5429261 Ki = 1.0 nM 9.00
CHEMBL5436775 Ki = 1.1 nM 8.96
CHEMBL3979547 Ki = 1.1 nM 8.96
CHEMBL3950572 Ki = 1.4 nM 8.85
CHEMBL5405655 Ki = 1.5 nM 8.82
CHEMBL5410262 Ki = 1.7 nM 8.77
CHEMBL3892073 Ki = 1.8 nM 8.74
CHEMBL3895570 Ki = 2.6 nM 8.59
CHEMBL5429002 Ki = 4.5 nM 8.35
CHEMBL3910265 Ki = 6.7 nM 8.17
CHEMBL5425858 Ki = 7.0 nM 8.15
CHEMBL5399050 Ki = 13.0 nM 7.89
CHEMBL3902752 Ki = 15.0 nM 7.82
CHEMBL3981623 Ki = 19.0 nM 7.72
CHEMBL3926904 Ki = 40.0 nM 7.40