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Assay Detail

CHEMBL5329844

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of TEAD2 (217 to 447 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells/FAM-labelled YAP1 (60 to 99 residues)(unknown origin) protein-protein interaction preincubated for 24 hrs at 4 degree C with TEAD1 followed by fluorescent labelled YAP1 addition by fluorescence polarization assay
8
Total Activities
8
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

TEAD2-YAP1 (CHEMBL5465558)
Type PROTEIN-PROTEIN INTERACTION
Organism Homo sapiens

Publication

Chloroacetamide fragment library screening identifies new scaffolds for covalent inhibition of the TEAD·YAP1 interaction.
Bum-Erdene K, Ghozayel MK, Zhang MJ, Gonzalez-Gutierrez G, Meroueh SO.
RSC Med Chem (2023) 14 : 1803 -1816
PubMed 37731696 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 4.63 5.64
Inhibition 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5423389 1 5.64
CHEMBL5421684 1 5.51
CHEMBL5437226 1 4.66
CHEMBL5431273 1 4.33
CHEMBL5420439 1 4.06
CHEMBL5429883 1 3.60
CHEMBL1719036 1 -
CHEMBL5428328 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5423389 IC50 = 2300.0 nM 5.64
CHEMBL5421684 IC50 = 3100.0 nM 5.51
CHEMBL5437226 IC50 = 22000.0 nM 4.66
CHEMBL5431273 IC50 = 46500.0 nM 4.33
CHEMBL5420439 IC50 = 86700.0 nM 4.06
CHEMBL5429883 IC50 = 254000.0 nM 3.60
CHEMBL1719036 Inhibition - % -
CHEMBL5428328 Inhibition - % -