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Assay Detail

CHEMBL5333105

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human Cathepsin B using Cbz-Phe-Arg-7-amino-4-methylcoumarin as substrate assessed as inhibition constant measured for 10 mins by fluorescence assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cathepsin B (CHEMBL4072)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-based lead optimization of peptide-based vinyl methyl ketones as SARS-CoV-2 main protease inhibitors.
Previti S, Ettari R, Calcaterra E, Di Maro S, Hammerschmidt SJ, Müller C, Ziebuhr J, Schirmeister T, Cosconati S, Zappalà M.
Eur J Med Chem (2023) 247 : 115021 -115021
PubMed 36549112 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 4.97 5.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5423619 1 5.10
CHEMBL5401213 1 4.96
CHEMBL5436027 1 4.84

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5423619 Ki = 7880.0 nM 5.10
CHEMBL5401213 Ki = 11100.0 nM 4.96
CHEMBL5436027 Ki = 14400.0 nM 4.84