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Assay Detail

CHEMBL5333941

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of UppP in Escherichia coli using FPP as substrate pretreated for 20 mins followed by FPP addition and measured after 60 mins by malachite green based assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Undecaprenyl-diphosphatase (CHEMBL4295581)
Type SINGLE PROTEIN
Organism Escherichia coli (strain K12)

Publication

Investigation into the Mechanism of Action of the Tuberculosis Drug Candidate SQ109 and Its Metabolites and Analogues in Mycobacteria.
Malwal SR, Mazurek B, Ko J, Xie P, Barnes C, Varvitsiotis C, Zimmerman MD, Olatunji S, Lee J, Xie M, Sarathy J, Caffrey M, Strynadka NCJ, Dartois V, Dick T, Lee BNR, Russell DG, Oldfield E.
J Med Chem (2023) 66 : 7553 -7569
PubMed 37235809 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 4.57 4.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1087059 1 4.82
CHEMBL5395162 1 4.80
CHEMBL5432995 1 4.75
CHEMBL3261880 1 4.68
CHEMBL561057 SQ109 2.0 1 4.50
CHEMBL5403488 1 4.43
CHEMBL3261881 1 4.04
CHEMBL5414003 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1087059 IC50 = 15000.0 nM 4.82
CHEMBL5395162 IC50 = 16000.0 nM 4.80
CHEMBL5432995 IC50 = 18000.0 nM 4.75
CHEMBL3261880 IC50 = 21000.0 nM 4.68
CHEMBL561057 SQ109 IC50 = 32000.0 nM 4.50
CHEMBL5403488 IC50 = 37000.0 nM 4.43
CHEMBL3261881 IC50 = 91000.0 nM 4.04
CHEMBL5414003 IC50 = 153000.0 nM -