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Assay Detail

CHEMBL5334710

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inverse agonist activity at human 5-HT2A receptor expressed in cell membrane assessed as increase in [35S]GTPgammaS binding by liquid scintillation spectrometry analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Subcellular Cell membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 2A (CHEMBL224)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, Molecular Docking, and Biological Evaluation of Novel Pimavanserin-Based Analogues as Potential Serotonin 5-HT<sub>2A</sub> Receptor Inverse Agonists.
Albujuq NR, Meana JJ, Diez-Alarcia R, Muneta-Arrate I, Naqvi A, Althumayri K, Alsehli M.
J Med Chem (2023) 66 : 9057 -9075
PubMed 37378639 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 7.30 7.90

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2111101 PIMAVANSERIN 4.0 1 7.90
CHEMBL5414080 1 7.10
CHEMBL5414859 1 7.10
CHEMBL5403965 1 7.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2111101 PIMAVANSERIN IC50 = 12.6 nM 7.90
CHEMBL5414080 IC50 = 79.4 nM 7.10
CHEMBL5414859 IC50 = 79.4 nM 7.10
CHEMBL5403965 IC50 = 79.4 nM 7.10