Assay Detail
Binding
CHEMBL5336081
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of full-length recombinant human NAMPT (2 to 491 residues) expressed in Escherichia coli using NAM as substrate preincubated for 5 mins followed by substrate addition measured after 15 mins by fluorescence based assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Nicotinamide phosphoribosyltransferase (CHEMBL1744525) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Synthesis and structure-activity relationship of new nicotinamide phosphoribosyltransferase inhibitors with antitumor activity on solid and haematological cancer.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 8.06 | 8.48 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL566757 | DAPORINAD | 2.0 | 1 | 8.48 |
| CHEMBL5428441 | — | — | 1 | 8.46 |
| CHEMBL5416318 | — | — | 1 | 8.30 |
| CHEMBL5421145 | — | — | 1 | 7.91 |
| CHEMBL5396894 | — | — | 1 | 7.16 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL566757 | DAPORINAD | IC50 | = | 3.27 | nM | 8.48 |
| CHEMBL5428441 | — | IC50 | = | 3.5 | nM | 8.46 |
| CHEMBL5416318 | — | IC50 | = | 4.98 | nM | 8.30 |
| CHEMBL5421145 | — | IC50 | = | 12.45 | nM | 7.91 |
| CHEMBL5396894 | — | IC50 | = | 69.1 | nM | 7.16 |