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Assay Detail

CHEMBL5341624

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiproliferative activity against mouse B16-F10 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type B16-F10
Confidence 1 — Organism
Curated By Autocuration

Target

B16-F10 (CHEMBL612656)
Type CELL-LINE
Organism Mus musculus

Publication

X-ray Crystal Structure-Guided Discovery of Novel Indole Analogues as Colchicine-Binding Site Tubulin Inhibitors with Immune-Potentiating and Antitumor Effects against Melanoma.
Ren Y, Wang Y, Liu J, Liu T, Yuan L, Wu C, Yang Z, Chen J.
J Med Chem (2023) 66 : 6697 -6714
PubMed 37145846 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 6.91 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5415595 1 8.70
CHEMBL5422119 1 7.75
CHEMBL5417734 1 7.68
CHEMBL4852240 1 7.54
CHEMBL5394538 1 7.50
CHEMBL5408636 1 7.48
CHEMBL5431034 1 7.37
CHEMBL5408121 1 7.22
CHEMBL5429479 1 7.18
CHEMBL107 COLCHICINE 4.0 1 7.06
CHEMBL4862445 1 7.01
CHEMBL5415401 1 6.86
CHEMBL5430075 1 6.71
CHEMBL5436501 1 6.70
CHEMBL5435747 1 6.62
CHEMBL5397132 1 6.57
CHEMBL5436288 1 6.51
CHEMBL5412258 1 6.41
CHEMBL5432944 1 6.31
CHEMBL5403422 1 6.20
CHEMBL5406755 1 6.17
CHEMBL5428736 1 6.00
CHEMBL5438931 1 5.29

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5415595 IC50 = 2.0 nM 8.70
CHEMBL5422119 IC50 = 18.0 nM 7.75
CHEMBL5417734 IC50 = 21.0 nM 7.68
CHEMBL4852240 IC50 = 29.0 nM 7.54
CHEMBL5394538 IC50 = 32.0 nM 7.50
CHEMBL5408636 IC50 = 33.0 nM 7.48
CHEMBL5431034 IC50 = 43.0 nM 7.37
CHEMBL5408121 IC50 = 60.0 nM 7.22
CHEMBL5429479 IC50 = 66.0 nM 7.18
CHEMBL107 COLCHICINE IC50 = 88.0 nM 7.06
CHEMBL4862445 IC50 = 98.0 nM 7.01
CHEMBL5415401 IC50 = 138.0 nM 6.86
CHEMBL5430075 IC50 = 197.0 nM 6.71
CHEMBL5436501 IC50 = 199.0 nM 6.70
CHEMBL5435747 IC50 = 237.0 nM 6.62
CHEMBL5397132 IC50 = 271.0 nM 6.57
CHEMBL5436288 IC50 = 310.0 nM 6.51
CHEMBL5412258 IC50 = 386.0 nM 6.41
CHEMBL5432944 IC50 = 492.0 nM 6.31
CHEMBL5403422 IC50 = 631.0 nM 6.20
CHEMBL5406755 IC50 = 683.0 nM 6.17
CHEMBL5428736 IC50 = 1004.0 nM 6.00
CHEMBL5438931 IC50 = 5169.0 nM 5.29