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Assay Detail

CHEMBL5342877

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Skp2 in human MGC-803 cells treated with shRNA 5'-GGACCTATCGAACTCAGTTAT-3' incubated upto 48 hrs by Western blot analysis (Rvb = 7 +/- 1.1 microM)
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MGC-803
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

S-phase kinase-associated protein 2 (CHEMBL3632458)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Novel 1,3-Diphenylpyrazine Derivatives as Potent S-Phase Kinase-Associated Protein 2 (Skp2) Inhibitors for the Treatment of Cancer.
Zhang K, Hu K, Li Q, Li M, Gao K, Yang K, Zhao B, Shi XJ, Zhang L, Liu HM.
J Med Chem (2023) 66 : 7221 -7242
PubMed 37204466 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 4.41 4.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5410031 1 4.41

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5410031 IC50 = 39200.0 nM 4.41