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Assay Detail

CHEMBL5346221

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human hormone nonresponsive PC-3 cells assessed as reduction in cell proliferation by MTT assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type PC-3
Confidence 1 — Organism
Curated By Autocuration

Target

PC-3 (CHEMBL390)
Type CELL-LINE
Organism Homo sapiens

Publication

A Homodimer of Withaferin A Formed by Base-Promoted Elimination of Acetic Acid from 27-<i>O</i>-Acetylwithaferin A Followed by a Diels-Alder Reaction.
Wijeratne EMK, Xu YM, Padumadasa C, Astashkin AV, Gunatilaka AAL.
J Nat Prod (2024) 87 : 583 -590
PubMed 38414352 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 6.31 6.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5401906 1 6.70
CHEMBL5406288 1 6.70
CHEMBL2047407 1 6.52
CHEMBL5403691 1 6.40
CHEMBL53463 DOXORUBICIN 4.0 1 6.22
CHEMBL5404628 1 6.00
CHEMBL5408627 1 5.66

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5401906 IC50 = 200.0 nM 6.70
CHEMBL5406288 IC50 = 200.0 nM 6.70
CHEMBL2047407 IC50 = 300.0 nM 6.52
CHEMBL5403691 IC50 = 400.0 nM 6.40
CHEMBL53463 DOXORUBICIN IC50 = 600.0 nM 6.22
CHEMBL5404628 IC50 = 1000.0 nM 6.00
CHEMBL5408627 IC50 = 2200.0 nM 5.66