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Assay Detail

CHEMBL5351364

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of pre-miR-21 (unknown origin) dicer cleavage activity preincubated for 30 mins followed by RNAase III addition and measured after 30 to 60 mins by FRET method
11
Total Activities
11
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Not specified
Confidence 3 — Cell-line / Tissue
Curated By Autocuration

Target

microRNA 21 (CHEMBL1615322)
Type NUCLEIC-ACID
Organism Homo sapiens

Publication

Synthesis of Bleomycin-Inspired RNA Ligands Targeting the Biogenesis of Oncogenic miRNAs.
Maucort C, Bonnet M, Ortuno JC, Tucker G, Quissac E, Verreault M, Azoulay S, Di Giorgio C, Di Giorgio A, Duca M.
J Med Chem (2023) 66 : 10639 -10657
PubMed 37449818 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 4.82 5.79
Inhibition 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5422790 1 5.79
CHEMBL5413573 1 5.43
CHEMBL5417854 1 4.93
CHEMBL5405932 1 4.79
CHEMBL5406620 1 4.69
CHEMBL5424223 1 4.69
CHEMBL5393793 1 4.51
CHEMBL5406161 1 4.42
CHEMBL5438709 1 4.12
CHEMBL5428791 1 -
CHEMBL5428849 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5422790 IC50 = 1620.0 nM 5.79
CHEMBL5413573 IC50 = 3740.0 nM 5.43
CHEMBL5417854 IC50 = 11800.0 nM 4.93
CHEMBL5405932 IC50 = 16400.0 nM 4.79
CHEMBL5406620 IC50 = 20300.0 nM 4.69
CHEMBL5424223 IC50 = 20300.0 nM 4.69
CHEMBL5393793 IC50 = 31200.0 nM 4.51
CHEMBL5406161 IC50 = 38300.0 nM 4.42
CHEMBL5438709 IC50 = 75900.0 nM 4.12
CHEMBL5428791 IC50 = 173000.0 nM -
CHEMBL5428849 Inhibition - % -